Juul Mint has joined Mango, Cucumber, Cream Brulee and Fruit Medley in the ashcan of history.The most fanciful sounding vape juice flavors are often high VG, low nicotine formulations that are optimized for use in a box mod.Cool, sweet and muted, Juul Mint changed vaping forever.It also makes for a great Juul Mint replacement because it is offered in both 25mg and 50mg nic strengths.Vapers that recently have been using menthol combustible cigarettes will enjoy the compact form factor of this disposable vape.There is no less expensive way to enjoy the best mint nic salts than in a refillable vape pod kit.A bottle of nic salt vape juice will typically cost less than 20 dollars and is equivalent to about 40 Juul pods.It has a touch of added sweetness to the powerful mint flavor that makes it a great “all-day” vape. .

Effects of mango and mint pod-based e-cigarette aerosol inhalation

Essential revisions: This is a potentially important paper that aimed to investigate the effects of vaping on multiple organs.A major limitation is the lack of investigation of (causal) pathophysiological consequences/general organ outcomes that might be driven by the reposted inflammatory response.We appreciate the reviewer identifying it as “of high interest and timely.” Of note, part of working in a new field such as e-cigarettes involves assessing for and detecting signals of potential clinical relevance.To bring the focus back to gene expression, we have conducted RNAseq on the lungs of JUUL exposed mice, and have included those data herein (new Figure 6) to highlight the effects of e-cigarette aerosols on gene expression in the lung, with a particular focus on differences between Mint and Mango flavors (the most popular JUUL flavors at the time of this study).These data support the hypothesis that e-cigarette aerosol inhalation fundamentally alters the lung, which raises the specter of downstream health effects.We have added further discussion regarding the potential consequences of inflammation in each of the organs (pages 13-18), with an emphasis on the effects of neuroinflammation on behavior and psychology.We have added the lack of dedicated behavioral and psychological evaluations as a limitation of this work and as an opportunity for discovery in future studies (page 19).We have conducted the mean linear intercept (MLI) measurements on e-cigarette aerosol exposed lungs and controls per ATS guidelines and have added these data to the manuscript (new Appendix 1- Figure 4M).Of note, the MLI data matches our original physiologic assessments of lung function (Appendix 1 – Figure 2A-2J), including elastance and compliance, which are known to change in the setting of emphysema.We set the target number of mice (n) at that time, such that these studies would be powered to detect a 25% change in cytokine expression.Based on discussions with our biostatisticians, we came to the conclusion that it would not be statistically appropriate to run more mice to increase the n when our primary outcome remains the same.Whilst I do not think that the low N's change the ultimate conclusions, but more rigor (ie more N's) would help solidify the paper given that it will likely be of great interest and scrutinized by the scientific community.We set the target number of mice (n) at that time, such that these studies would be powered to detect a 25% change in cytokine expression.We did go through and reviewed all of the data with our biostatisticians, we came to the conclusion that it would not be statistically appropriate to run more mice to increase the n when our primary outcome remains the same.We have conducted the mean linear intercept (MLI) measurements on e-cigarette aerosol exposed lungs and controls per ATS guidelines and have added these data to the manuscript (new Appendix 1- Figure 4M).Of note, the MLI data matches our original physiologic assessments of lung function (Appendix 1 – Figure 2A-2J), including elastance and compliance, which are known to change in the setting of emphysema.One of the must novel conclusions from this paper is increased inflammation in the brain which the authors speculate could lead to altered moods and or change the addiction threshold.We have added further discussion regarding the potential consequences of inflammation in each of the organs (pages 13-19), with an emphasis on the effects of neuroinflammation on behavior and psychology.We have added the lack of dedicated behavioral and psychological evaluations as a limitation of this work and as an opportunity for discovery in future studies (page 19-20).Reviewer #2: Under homeostasis conditions, the authors observed sign of inflammatory responses in the brain, the heart and the colon, while no inflammation was detected in the broncho-alveolar lavage fluid of the mice following exposures to JUUL aerosols.Also, JUUL aerosol exposures mediated airway inflammatory responses in the acute lung injury model (LPS).In late 2019 the FDA was moving to ban specific flavors for pod devices, which include those for Mint and Mango.We have expanded the limitation section within the Discussion, and have pointed out that because these studies were conducted in female mice alone, the results may not represent effects in males.Although there are a few limitations related to this study, which should be included in the manuscript, overall, the authors' claims and conclusions are based on the data that is presented through multiple figures.The new title is now: “Effects of Mango and Mint pod-based e-cigarette aerosol inhalation on inflammatory states of the brain, lung, heart and colon in mice”.– In the introduction and Discussion sections, the authors should consider including all in vitro and in vivo studies that have been conducted on JUUL aerosols, since there are very few of those already published articles.The literature review found a total of 13 articles all of which have been cited in the revised manuscript, primarily via the following paragraph added to the Introduction:.Introduction: “Studies of JUUL to date have been predominantly subacute and acute exposures with a focus on in vitro and in vivo experiments.A majority of the current literature examined the cytotoxic effects of varying JUUL flavors (Crème Brulee, Cool Mint, Fruit Medley, Tobacco, Menthol, etc.).These studies concluded that while JUUL pods had significantly lower oxidant yields in comparison to combustible cigarettes, the nicotine concentrations were substantially greater (18-20).While these studies have laid the groundwork for assessing the acute impact of JUUL use on the respiratory system, much work remains to be done on the effects across the body.”.But because many changes could be occurring at the gene expression level, that could set the stage for pathologic responses to common clinical challenges, we conducted new RNAseq studies on lung tissues and have included these data as new Figure 6.We do plan on running JUUL exposures in male mice, but these will be limited to Tobacco and Menthol flavors since these are currently the only ones available on the market.Reviewer #3: In this study, Alex Moshensky et al., investigated effects of chronic aerosol inhalation of flavored JUUL on inflammatory markers in several organs, including brain, lung, heart, and colon in a mouse model.They found that JUUL inhalation upregulated a number of cytokine and chemokine gene expression and increased HMGB1 and RAGE in the nucleus accumbens.To bring the focus back to gene expression, we have conducted RNAseq on the lungs of JUUL exposed mice, and have included those data herein to highlight the effects of e-cigarette aerosols on gene expression in the lung, with a particular focus on differences between Mint and Mango flavors (the most popular JUUL flavors at the time of this study).These new data (new Figure 6) support the hypothesis that e-cigarette aerosol inhalation fundamentally alters the lung, which raises the specter of downstream health effects.Although the formulation of JUUL e-liquids is proprietary, their website claims simplicity (https://www.juul.com/learn/pods) in that they use pharmaceutical grade propylene glycol and glycerol (which makes up the majority of their e-liquids), in order to form an aerosol which carries pharmaceutical grade nicotine and benzoic acid (when combined, create a nicotine salt), and flavors (which can be can be a mixture of natural and artificial ingredients).In Dr. Kheradmand’s recent work, mice exposed to e-cigarette aerosols with and without nicotine were much more susceptible to acute lung injury in the setting of viral pneumonia.Further work is needed to truly define how the subtle, broad shifts in the cytokine milieu across organs will impact the health of e-cigarette vapers.For example, Madison et al., exposed mice to e-cigarette aerosols with and without nicotine and found that both exposures increased susceptibility to acute lung injury in the setting of viral pneumonia.Further work is needed to truly define how the subtle, broad shifts in the cytokine milieu across organs will impact the health of e-cigarette vapers.”.To fully evaluate the health impact of evolving cigarette, it would be informative to included other tobacco or vaping device as control.We agree that such comparisons are likely to provide insight into the differences between devices and formulations and versus cigarette smoke, and thus will be incredibly important for the field.However, because we have run parallel studies in the past with vape pens, box mods, and conventional tobacco, we are hopeful to start combining data to look for trends and differences across inhalant exposures.Although we were unable to include protein based quantification for all organs, we do feel that our original intention of demonstrating broad reaching effects of e-cigarette aerosols across the body was still achieved.But we absolutely agree that more work is needed to fully define the impact of e-cigarette use at the protein, cellular, and organ level.

.

High levels of cancer-causing chemical found in mint and menthol e

The researchers used previously published Centers for Disease Control data of the concentrations of pulegone in select e-cigarette liquids -- and also in smokeless tobacco -- and combined these with the ingestion exposure data from animal studies to calculate a “margin of exposure,” a level used by regulatory agencies including the FDA to assess human health risks.Theoretically, the larger the safety margin, the safer the product.“We found that for all mint and menthol liquids that we analyzed, the range was 300-6,000, where levels should be at least above 10,000,” Dr. Sairam Jabba, Senior Research Associate at Duke University and co-author of the study, told ABC News.Brands evaluated by the study included V2 -- a company later purchased by JUUL -- as well as Premium, South Beach Smoke and Skoal mint snuff.“With pulegone known to be a potential human carcinogen, and in the background of youth epidemic and the recent hospitalizations of e-cigarette users, relying on the absence of respiratory data is irresponsible,” said Jabba.Earlier this year, researchers from the University of California at Riverside published an extensive chemical analysis of JUUL liquids, which found detectable levels of pulegone in “Cool Mint” and “Classic Menthol” flavors.“Based on the calculation in the study, in order to reach an exposure level above the threshold of carcinogenic concern for pulegone, a person would have to consume hundreds of pods per day.”.Lauren Kelly, MD, MPH, is an Internal Medicine resident physician in New York City working with the ABC News Medical Unit. .

JUUL and JUULpod Alternatives to Consider – Price Point NY

Competition is just as good for the vaping industry, as it is within others too, and it also assists in ensuring higher quality products.As JUUL undergoes some transitions, one of them is a problem with the FDA and another is their new partnership with Altria, there might be a decline in the availability of the pods, more specifically, the fruit flavored variety.The battery life of the JUUL has been the source of much annoyance (200mAH), with other vape pens offering a longer smoke time between charges.When people seek JUUL alternative products, they are looking for no tanks, no buttons, and importantly no hassles.The device is fully charged within 20 minutes of connecting to a USB charger and offers up to 100 satisfying puffs.The MYLE is proving to be very popular closed system salt nic vaping device and definitely has a fair chance to compete against JUUL.The MYLE starter kit ,available in Royal Blue and Midnight Black, includes the device, a micro- USB charger, and four different pod flavors.The device is available in a variety of colors including Midnight Black, Gun Metal, Prime Pink, Elite White and Royal Blue.Its ergonomic design is aesthetically pleasing and it is user friendly while offering smooth inhalation.Flavors available: Summer Strawberry, Lush Ice, Mighty Mint, Cubano, and Tropical Mango.With satisfying vapor production and a sufficient throat hit, a single device has approximately 270 puffs before running empty.Flavors available: Strawberry, Mango, Watermelon, Banana, Lemonade, Green Apple, Blue Raspberry, and California Cream.A little insider trick, try taking a hit when covering up on of the holes on the top of the pod to get a whole different experience ;). .

Air Factory

This is a sweet spearmint and peppermint flavor, a combination which is perfect for those who want to have that menthol taste without the harsh menthol-y sensation.This versatile refillable vape pod kit can also handle the 70 percent VG Air Factory E-Liquids. .

Puff Plus Cool Mint

Icier and a bit less spearminty than the competition, adult menthol, and mint vapers will love the staying and hitting power of this Puff Plus. .

E H J A P

Leave a reply

your email address will not be published. required fields are marked *

Name *
Email *
Website